Fatemeh Hendijani; Jamal Fayazi; Hedayat-allah Roshanfekr; Mohammad Reza Ghorbani
Volume 24, Issue 3 , October 2022, , Pages 271-279
Abstract
In order to investigate the effect of vitamin E and vitamin E nanoliposomes on the expression of the STAR gene in testis and TSPO gene in the ovary of Japanese quail, an experiment using 864 pieces of quail for 10 weeks in a completely randomized design with six treatments, six replications and ...
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In order to investigate the effect of vitamin E and vitamin E nanoliposomes on the expression of the STAR gene in testis and TSPO gene in the ovary of Japanese quail, an experiment using 864 pieces of quail for 10 weeks in a completely randomized design with six treatments, six replications and 24 Breeding quail (16 females and eight males) was performed in each replication. The treatments were fed diets containing different levels of vitamin E (25, 50 and 100 IU per kg of diet) and vitamin E nanoliposomes (25, 50 and 100 IU per kg of diet). The results of this study showed that the effect of the experimental treatments on the expression of TSPO gene in the ovary and STAR gene in the testis was significant (P<0.05). The addition of 50 IU of vitamin E significantly increased TSPO gene expression in the ovary compared to the control treatment (P<0.05). The results also showed that the level of 25 IU nanoliposome of vitamin E increased the expression of TSPO gene in the ovary, which was not significantly different from the control treatment. The use of vitamin E and vitamin E nanoliposome levels significantly decreased the expression of STAR gene in the testis of Japanese quail (P<0.05). According to the results of this study, the addition of 25 IU vitamin E nanoliposomes as well as 50 and 100 IU levels of vitamin E has a significant effect on the expression of TSPO gene in the ovary, which is one of the genes affecting fertility and reproduction.
Maryam Karami; Karim Hasanpur; Jamal Fayazi; Arash Javanmard; Hamid Varnaseri
Volume 23, Issue 4 , January 2022, , Pages 501-514
Abstract
The aim of present study was to investigate the effect of selection against ascites syndrome on different traits body growth, internal organs, and blood parameters in a broiler line. Therefore, by classifying different families based on the frequency of ascites under intense ascites inducing condition ...
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The aim of present study was to investigate the effect of selection against ascites syndrome on different traits body growth, internal organs, and blood parameters in a broiler line. Therefore, by classifying different families based on the frequency of ascites under intense ascites inducing condition (AIC), 10 susceptible families (SUS) and 10 resistant families (RES) to ascites were selected and another set of offspring from the same families were bred under normal commercial condition (NCC). This process of selection and cross validation was carried out twice in the two distinct generations. The genetic relationship of different traits, the difference in the frequency of ascites between AIC and NCC, and the difference between different traits in SUS and RES were performed by correlation statistics, Chi-square test, and GLM procedure of SAS software (version 9.1), respectively. The correlation between ascites susceptibility in different families indicated that families that showed more ascites under AIC also had more ascites under NCC. Comparative study of different traits in susceptible and resistant groups showed that in SUS, body weight, growth rate and respiratory capacity were significantly lower and blood partial pressure of carbon dioxide was significantly higher than RES (P<0.05). In the present study it was shown that selection against this syndrome not only does not reduce growth rate in the investigated broiler line, but also improves growth traits and resistance to this syndrome in the mentioned line.
Bizhan Mahmoudi; Hedayatollah Roshanfekr; Mohsen Sari; Mohammad Reza Bakhtiarizadeh
Volume 22, Issue 3 , September 2020, , Pages 337-348
Abstract
The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine ...
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The objective of this study was to identify known intergenic lncRNAs related to biological pathways of acidosis in Holstein calves using ruminaltissue. Two groups of healthy calves (N=3) and affected by acidosis (N=3) were compared. Paired-end sequencing method was performed using theHiseq2500 illumine platform. Hisat2 software was used to align reads to the bovine reference genome and StringTie software package was used toassemble read files into transcripts. Using next generation sequencing, 1636 genes belonging to known intergenic lncRNAs were identified, of which56 genes showed significant differential expression (P≤0.05). Neighbor genes of known intergenic lncRNAs were determined on bovine genome.Analysis of biological pathways and molecular function showed that five biological pathways were significantly (P≤0.05) enriched. These pathwayswere Apelin signaling pathway, Gap junction, Glucagon signaling pathway, Renin secretion, and AGE-RAGE signaling pathway. Moreover, twomolecular functions including gap junction channel activity, and phosphatidyl inositol phospholipase C activity were significantly (P≤0.05) enriched.Some lncRNAs have different expression in healthy and acidosis samples, and the decreased pH acts as a stimulus to activate some biologicalsignaling pathways. In conclusion, it was indicated that lncRNAs with differential expression between the control group and the group affected byacidosis are associated with pathways related to rumen energy metabolism and signaling. Identified differentially expressed lncRNAs could be used asprognostic in acidosis and biomarkers or promising candidates in animal breeding.
Maryam Bazgiri; Mohammad taghi Beigi nassiri; Jamal Fayazi
Volume 19, Issue 1 , May 2017, , Pages 13-22
Abstract
This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with ...
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This study was conducted to investigate the Single nucleotide polymorphism of STAT1 gene by PCR-RFLP technique and association of genotypes with milk production traits in Brown Swiss dairy cattle. The blood samples of ninety-eight cows were collected randomly from tail vein. The DNA was extracted with Cinagene kit (PR881612C). Fragment of 314 bp in 3'UTR of STAT1 gene was amplified by PCR and digested by Pag1 restriction enzyme. Genotype frequencies were calculated by PopGene software (version 1.31). The SAS 9.1 software was used for statistical analysis and the mean of traits was compared by Duncan test. The genotypefrequencies of TT, TC and CC were 0.23, 0.25 and 0.52, respectively. The T and C allele frequency were 0.355 and 0.645, respectively. The expected heterozygosity was estimated 0.458. Result of Chi-square showed that this locus was not in the Hardy Weinberg equilibrium (P<0.05). The means of traits for milk, fat percent and fat yield, and protein yield were not significant but means of protein percent was significant (P<0.05). The mean of milk, fat and protein yield traits for CC genotype were more than two other genotypes. The results of present study showed that T allele is known as minor frequency allele and C allele have positive effect on milk, fat and protein yield traits.
Fatemeh Jafari; Mohammad Taghi Beige Nasiri; Jamal Fayazi; Mohammad Ali Loghavi
Volume 17, Issue 2 , October 2015, , Pages 171-182
Abstract
Genetic parameters direct and maternal in Fars native flows using the Bayesian statistical approach, was estimation with consider body weight at a day of age (BW1), body weight at eight weeks of age (BW8), body weight at 12 weeks of age (BW12), age at sexual maturity (ASM), egg number (EGP) and mean ...
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Genetic parameters direct and maternal in Fars native flows using the Bayesian statistical approach, was estimation with consider body weight at a day of age (BW1), body weight at eight weeks of age (BW8), body weight at 12 weeks of age (BW12), age at sexual maturity (ASM), egg number (EGP) and mean egg weight during 28th, 30th and 32nd week ages (AV283032) Fars native flows. Genetic parameters were estimated by six different animal models and MTGSAM software. The best model was determined using the Akaike information criterion for each trait. The direct heritability (h2) estimates basis best model BW1, BW8, BW12, ASM, EGP and AV283032 0.36, 0.33, 0.30, 0.48, 0.26 and 0.25, respectively. Maternal effects were significant for ASM, EGP and AV283032 traits studied and estimates of maternal environmental variance as a proportion of phenotypic variance (c2) varied from 0.01 (EGP and AV283032) to 0.39 (BW1). Results present research showed that Bayesian approach by considering all factors more complicated models was selected as a model.
Elham Javdan; Jamal Fayazi; Saleh Tabatabaei; Mohammadtaghi Baigi Nasiri
Volume 15, Issue 2 , October 2013, , Pages 101-107
Abstract
This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, ...
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This study was conducted to detect polymorphism of gene BMP15, a member of Transforming GrowthFactor β (TGFβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. Samples were randomly selected from 91 Najdi goats in 3 geographicallocations, northwest, southeast and center of Khuzestan province. After DNA extraction, amplification of235 bp fragment of exon 2 of BMP15 gene was performed using specific primers. Sequence detectionwas executed after amplification of gene fragments. The association of BMP15 gene and litter size wasdone by SAS software. Results from sequencing were analyzed by Vector NTI software. The resultsidentified three mutations in bases 529 (T to G), 530 (C to G) and 576 (T to C). The largest litter sizebelongs to AA pattern. Point mutations in this gene will alter the Ovulation rate of the goat. Therefore,improving twining trait in Najdi population can be expected by marker assisted selection.